This study was undertaken to determine the influence ofAloe vera(AV) on changes induced in pulmonary tissue of cigarette smoke (CS) inhaling mice. CS inhalation for 4 weeks caused pulmonary damage as evident by histoarchitectural alterations and enhanced serum and tissue lactate dehydrogenase (LDH) activities. CS inhalation also led to increased mucin production as revealed by mucicarmine and Alcian Blue-Periodic Acid Schiff (AB-PAS) staining. Studies on bronchoalveolar lavage fluid (balf) of CS exposed animals revealed structural changes in phospholipids and increase in surface tension when compared with control counterparts. These changes were accompanied by enhanced nitric oxide (NO) levels, citrulline levels, peroxidative damage, and differential modulation ofantioxidantdefense system. AV administration (seven weeks, 500 mg/kg b.w. daily) to CS inhaling mice led to modulation of CS induced pulmonary changes as revealed by lesser degree of histoarchitectural alterations, lesser mucin production, decreased NO levels, citrulline levels, peroxidative damage, and serum LDH activity. AV treatment to CS inhaling mice was associated with varying response toantioxidantdefense system, however half of CS + AV treated animals did not exhibit appreciable changes when compared with that of CS exposed animals. These observations suggest that AV has the potential to modulate CS induced changes in the pulmonary tissue which could have implications in management of CS associated pulmonary diseases, however, further investigations are required to explore its complete mechanism of action.
Drug Chem Toxicol. 2014 Apr;37(2):135-43. doi: 10.3109/01480545.2013.834350. Epub 2014 Feb 13.
Evaluation of in vitro and in vivo antioxidant potential of polysaccharides from Aloe vera (Aloebarbadensis Miller) gel.
Abstract In the present study, theantioxidantactivity of the polysaccharides fromaloe vera(Aloebarbadensis Miller) gel was evaluated, in vitro by five established methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH(-)) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide scavenging, superoxide radical (O(-2)) scavenging and reducing power assay, and in vivo against doxorubicin (DOX)-induced myocardial oxidative stress (OS) in albino wistar rats. The polysaccharides exhibited significant inhibitory activity against DPPH(-), superoxide, NO and hydrogen peroxide scavenging assay with significant reducing activity at all concentrations used. DOX-induced (7.5 mg/kg, intravenously) cardiotoxicity manifested biochemically by a significant decrease in blood and tissue glutathione (GSH) along with elevated levels of serum lactate dehydrogenase and creatine phosphokinase. In addition, cardiotoxicity was further confirmed by the significant increase in lipid peroxidation expressed as thiobarbituric acid reactive substances (TBARS), catalase (CAT) and superoxide dismutase (SOD).
Administration ofaloe verapolysaccharides for 14 days produced a marked protection against cardiotoxicity induced by DOX evidenced by significant reductions in serum lactate dehydrogenase, serum creatine phosphokinase, cardiac TBARS, CAT and SOD along with increased levels of blood and tissue GSH in a dose-dependent manner. The present investigation is the first to establish theantioxidantpotency of the polysaccharides fromaloe veraagainst DOX-induced myocardial OS.
The aim of this study was to identify, quantify, and compare the phytochemical contents, antioxidantcapacities, and antibacterial activities of Aloe vera lyophilized leaf gel (LGE) and 95% ethanol leaf gel extracts (ELGE) using GC-MS and spectrophotometric methods.
Analytically, 95% ethanol is less effective than ethyl acetate/diethyl ether or hexane (in the case of fatty acids) extractions in separating phytochemicals for characterization purposes. However, although fewer compounds are extracted in the ELGE, they are approximately 345 times more concentrated as compared to the LGE, hence justifying ELGE use in biological efficacy studies in vivo. Individual phytochemicals identified included various phenolic acids/polyphenols, phytosterols, fatty acids, indoles, alkanes, pyrimidines, alkaloids, organic acids, aldehydes, dicarboxylic acids, ketones, and alcohols. Due to the presence of the antioxidant polyphenols, indoles, and alkaloids, the A. vera leaf gel shows antioxidantcapacity as confirmed by ORAC and FRAP analyses. Both analytical methods used show the non-flavonoid polyphenols to contribute to the majority of the total polyphenol content. Three different solvents such as aqueous, ethanol, and acetone were used to extract the bioactive compounds from the leaves of A. vera to screen the antibacterial activity selected human clinical pathogens by agar diffusion method. The maximum antibacterial activities were observed in acetone extracts (12 ± 0.45, 20 ± 0.35, 20 ± 0.57, and 15 ± 0.38 nm) other than aqueous and ethanol extracts.
Due to its phytochemical composition, A. vera leaf gel may show promise in alleviating symptoms associated with/or prevention of cardiovascular diseases, cancer, neurodegeneration, and diabetes.
Aloe vera is a semi-tropical plant of Liliaceae family which has a wide range of applications in traditional medicine. In the present study, we sought to investigate the heptaoprotective potential of Aloe vera gel as a diet supplement. To achieve this goal, we have designed in vitro and in vivo experimental models of chemical-induced liver damage using male Sprague-Dawley rat. In the in vitro model, its effect was evaluated on Fenton's reaction-induced liver lipid peroxidation. Co-incubation with gel significantly reduced the generation of liver lipid peroxide (LPO). Next, to see the similar effect in vivo, gel was orally administered to rats once daily for 21 successive days. Following 1 hour of the last administration of gel, rats were treated with intra-peritoneal injection of CCl4. Dietary gel showed significant hepatoprotection against CCl4-induced damage as evident by restoration of liver LPO, serum transaminases, alkaline phosphatase, and total bilirubin towards near normal. The beneficial effects were pronounced with the doses used (400 and 800 mg/kg body weight). Besides, we did not observe any significant drop in serum albumin, globulin as well as total protein levels of gel-administered rats. Histopathology of the liver tissue further supported the biochemical findings confirming the hepatoprotective potential of dietary gel.
Molecules. 2012 Nov 1;17(11):12851-67. doi: 10.3390/molecules171112851.
In vitro antioxidant effects of Aloe barbadensis Miller extracts and the potential role of these extracts as antidiabetic and antilipidemic agents on streptozotocin-induced type 2 diabetic model rats.
In this study, the total phenolic and flavonoid contents, the 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging ability and the ferric reducing power (FRAP) of Aloe vera were measured to determine the antioxidant activity of this species. The in vivo antidiabetic effects of the plant were also investigated using streptozotocin-induced type 2 diabetic model rats that were divided into five groups based on the treatment received: (1) water (WC); (2) glibenclamide; (3) concentrated gel extract (Gel-C); (4) ethanol (80%) gel extract (Gel-Et); and (5) ethanol (80%) skin extract of Aloe vera (Skin-Et). Skin-Et, which contained the highest level of total phenolics (62.37 ± 1.34 mg(gallic acid)/kg) and flavonoids (20.83 ± 0.77 mg/kg), exhibited the highest scavenging activity (85.01 ± 0.52%) and the greatest reducing power (185.98 ± 0.41 µM), indicating that the skin contained the highest level of antioxidants. The oral consumption of Gel-Et for 4 weeks a caused significant reduction in the fasting serum glucose levels of the rats. The rats in the Gel-C-, Gel-Et- and Skin-Et-treated groups experienced a reduction in their total cholesterol levels by 11%, 17% and 25%, respectively and a reduction in their LDL cholesterol levels by 45%, 3% and 69%, respectively. The in vivo experimental antioxidant parameter MDA is strongly correlated with the in vitro antioxidant parameters of flavonoids and polyphenols, namely the DPPH and FRAP values (r = 0.94, 0.92, 0.93, 0.90), thus confirming the antioxidant potential of the Aloe vera extracts.
Aloe preparations have maintaining their popularity over the of course time. Aloe latex is used for its laxative effects; aloe gel is used topically for skin ailments and internally for variety of disturbances; aloe extract is potentially useful for cancer.
Nutrition. 2013 Sep;29(9):1110-4. doi: 10.1016/j.nut.2013.02.015. Epub 2013 Jun 2.
Metabolic effects of aloe vera gel complex in obese prediabetes and early non-treated diabetic patients: randomized controlled trial.
The metabolic effects of an aloe vera gel complex (Aloe QDM complex) on people with prediabetes or earlydiabetes mellitus (DM) are unknown. The goal of this study was to determine the effects of Aloe QDM complex on body weight, body fat mass (BFM), fasting blood glucose (FBG), fasting serum insulin, and Homeostasis Model of Assessment - Insulin Resistance (HOMA-IR) in obese individuals with prediabetes or early DM who were not on diabetes medications.
Participants (n = 136) were randomly assigned to an intervention or a control group and evaluated at baseline and at 4 and 8 wk.
The study lost six participants in the control group and eight in the intervention group. At 8 wk, body weight (P = 0.02) and BFM (P = 0.03) were significantly lower in the intervention group. At 4 wk, serum insulin level (P = 0.04) and HOMA-IR (P = 0.047) were lower in the intervention group; they also were lower at 8 wk but with borderline significance (P = 0.09; P = 0.08, respectively). At 8 wk, FBG tended to decrease in the intervention group (P = 0.02), but the between-group difference was not significant (P = 0.16).
In obese individuals with prediabetes or early untreated DM, Aloe QDM complex reduced body weight, BFM, and insulin resistance.
Warts are most often confused with molluscum contagiosum. Molluscum is a common viral infection of the skin, more common in children. It is caused by a DNA virus of the poxvirus group. The lesions are discrete, pearly, skin-colored, dome-shaped papules varying in size from 1 to 5 mm. Typically they have central umbilication from which a plug of cheesy material can be expressed. These papules may occur anywhere, but the face, eyelids, neck, underarms, and thighs are the most common sites of infection. Lesions may also occur in clusters on the genitalia or in the groin of adolescents. Mucosal lesions are rare.
Molluscum contagiosum is a self-limited disease, but lesions can persist for months to years and can spread to distant sites, and may be transmitted to others. Molluscum contagiosum responds poorly to most surgical treatments such as curretage and cryotherapy since this does not shorten the duration of the disease, and it usually causes deep tissue scars.
There are two good therapy options. The cheapest is probably using Lugol's 5% iodine. Apply it to the lesions as they develop. Treat most lesions for 3-4 days, twice a day, with a small drop of Lugol's, then blot dry. And do this until it causes the lesion to become inflammed or uncomfortable to treat. Only treat new lesions and avoid treating facial lesions. Once immunity develops, the facial lesions will disappear naturally, so you can simply treat the non-facial lesions.
The other good option is to take a good aloe supplement orally to boost immunity, and apply the aloe to the lesion twice a day mixed with some vaseline or cream.
Both methods help the body develop immunity to the virus by exposing the virus to the immune system. The iodine is less expensive but sometimes more uncomfortable, but both methods generally produce the same final result in 2-3 months.
Try to discourage scratching of the lesions, and keep fingernails clean to limit transmission and spreading.
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GotPetsOnline™ reserves all rights not expressly granted in and to the GotPetsOnline™ Content and Services. 8.18 You agree to not engage in the use, copying, or distribution of any of the GotPetsOnline™ Content other than expressly permitted herein, including any use, copying, or distribution of user Content or that of third-parties obtained through the GotPetsOnline™ for any commercial purposes. 8.19 You agree not to circumvent, disable or otherwise interfere with security-related features of GotPetsOnline™ that prevent or restrict use or copying of any GotPetsOnline™ Content or enforce limitations on use of GotPetsOnline™ Content or Services. 8.20 You understand that when using GotPetsOnline™ Services, you will be exposed to user Content and other Content from a variety of sources, and that GotPetsOnline™ is not responsible for the accuracy, usefulness, safety, or intellectual property rights of or relating to such Content. 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Section 9: License 9.1 GotPetsOnline.com™ grants you a personal, worldwide, non-assignable, non-transferable, and non-exclusive license to use the Services provided to you by GotPetsOnline.com™. This license is for the sole purpose of enabling you to use and enjoy the benefit of the Services as provided by GotPetsOnline.com™, in the manner permitted by the Agreement. 9.2 Except as expressly authorized by GotPetsOnline.com™, you agree not to modify, rent, lease, loan, sell, distribute or create derivative works based on GotPetsOnline.com™ Services (including any software on which the Services are based). You agree not to modify the Services in any manner or form, or to use modified versions of the Services, including (without limitation) for the purpose of obtaining unauthorized access to our Services. 9.3 You shall not (and you shall not permit anyone else to) copy, modify, create a derivative work of, reverse engineer, reverse assemble, decompile or otherwise attempt to extract or discover the source code of the Services (or any part thereof), sell, assign, sublicense, grant a security interest in or otherwise transfer any right in the Services unless GotPetsOnline.com™ expressly permits you in writing or it is required by law. 9.4 Unless GotPetsOnline.com™ has given you specific written permission to do so, you shall not assign (or grant a sub-license of) your rights to use the Services, grant a security interest in or over your rights to use the Services, or otherwise transfer any part of your rights to use the Services.
Section 10: Content License 10.1 You retain copyright and any other rights you already hold in Content which you submit, post or display on or through, the Services. (a) By submitting, posting or displaying the Content you give GotPetsOnline.com™ a perpetual, irrevocable, worldwide, royalty-free, and non-exclusive License to reproduce, adapt, modify, translate, publish, publicly perform, publicly display and distribute any Content which you submit, post or display on or through, the Services. (b) This License is for the sole purpose of enabling GotPetsOnline.com™ to display, distribute and promote the Services. 10.2 You agree that this License includes a right for GotPetsOnline.com™ to make such Content available to other companies, organizations or individuals with whom GotPetsOnline.com™ has relationships for the provision of syndicated services, and to use such Content in connection with the provision of those Services. 10.3 You understand that GotPetsOnline.com™, in performing the required technical steps to provide the Services to our users, may take certain actions. You agree that this License shall permit GotPetsOnline.com™ to take the following actions. (a) Transmit or distribute your Content over various public networks and in various media. (b) Make such changes to your Content as are necessary to conform and adapt that Content to the technical requirements of connecting networks, devices, services or media. 10.4 With respect to Content other than photos, graphics, audio or video you submit or make available for inclusion on publicly accessible areas of our Service, you grant GotPetsOnline.com™ the perpetual, irrevocable and fully sub-licensable License to use, distribute, reproduce, modify, adapt, publish, translate, publicly perform and publicly display such Content and to incorporate such Content into other works in any format or medium now known or later developed. "Publicly accessible" areas of our Service are those areas that are intended by GotPetsOnline.com™ to be available to the general public. 10.5 You confirm and warrant to GotPetsOnline.com™ that you have all the rights, power and authority necessary to grant the above Content License.
Section 11: Proprietary Rights 11.1 You acknowledge and agree that GotPetsOnline.com™ owns all legal right, title and interest in and to the Services, including any intellectual property rights which subsist in the Services (whether those rights happen to be registered or not, and wherever in the world those rights may exist), including patent rights. You further acknowledge that the Services may contain information which is designated confidential by GotPetsOnline.com™ and that you shall not disclose such information without prior written consent from GotPetsOnline.com™. 11.2 Unless you have agreed otherwise in writing with GotPetsOnline™, nothing in the Agreement gives you a right to use any of GotPetsOnline.com™ trade names, trade marks, service marks, logos, domain names, and other Distinctive Features. 11.3 If you have been given an explicit right to use any of these Distinctive Features in a separate written agreement with GotPetsOnline.com™, then you agree that your use of such features shall be in compliance with that agreement, any applicable provisions of the Agreement, and GotPetsOnline.com™ Distinctive Feature use rules as updated from time to time. Rules for Third-Party Use of Distinctive Features is incorporated by reference herein. 11.4 Unless you have been expressly authorized to do so in writing by GotPetsOnline.com™, you agree that in using the Services, you will not use any trade mark, service mark, trade name, logo of any company or organization in a way that is likely or intended to cause confusion about the owner or authorized user of such marks, names or logos. 11.5 You agree that you shall not remove, obscure, or alter any proprietary rights notices (including copyright and trademark notices) which may be affixed to or contained within the Services. 11.6 Other than the limited license set forth in Section 10, GotPetsOnline.com™ acknowledges and agrees that it obtains no right, title or interest from you (or your licensors) under this Agreement in or to any Content that you submit, post, transmit or display on, or through, the Services, including any intellectual property rights which subsist in that Content (whether those rights happen to be registered or not, and wherever in the world those rights may exist). Unless you have agreed otherwise in writing with GotPetsOnline.com™, you agree that you are responsible for protecting and enforcing those rights and that GotPetsOnline.com™ has no obligation to do so on your behalf.
Section 12: Copyright and Trademark Policies 12.1 GotPetsOnline.com™ operates a trademark complaints procedure in respect to GotPetsOnline.com™ advertising business. This procedure is explained in the Trademark Complaint Procedure of GotPetsOnline.com™, and is incorporated by reference herein. 12.2 It is GotPetsOnline.com™ policy to respond to notices of alleged copyright infringement that comply with applicable international intellectual property law (including, in the United States, the Digital Millennium Copyright Act) and to terminate the accounts of repeat infringers. This policy is explained in the Copyright Infringement Policy and DMCA (Digital Millennium Copyright Act) of GotPetsOnline.com™, and is incorporated by reference herein.
Section 13: Exclusion of Warranties 13.1 NOTHING IN THESE TERMS, INCLUDING SECTIONS 13 AND 14, SHALL EXCLUDE OR LIMIT GOTPETSONLINE.COM™ WARRANTY OR LIABILITY FOR LOSSES WHICH MAY NOT BE LAWFULLY EXCLUDED OR LIMITED BY APPLICABLE LAW. (a) SOME JURISDICTIONS DO NOT ALLOW THE EXCLUSION OF CERTAIN WARRANTIES OR CONDITIONS OR THE LIMITATION OR EXCLUSION OF LIABILITY FOR LOSS OR DAMAGE CAUSED BY NEGLIGENCE, BREACH OF CONTRACT OR BREACH OF IMPLIED TERMS, OR INCIDENTAL OR CONSEQUENTIAL DAMAGES. (b) ACCORDINGLY, ONLY THE LIMITATIONS WHICH ARE LAWFUL IN YOUR JURISDICTION WILL APPLY TO YOU AND OUR LIABILITY WILL BE LIMITED TO THE MAXIMUM EXTENT PERMITTED BY LAW. 13.2 YOU EXPRESSLY UNDERSTAND AND AGREE THAT YOUR USE OF THE SERVICES IS AT YOUR SOLE RISK AND THAT THE SERVICES ARE PROVIDED "AS IS" AND "AS AVAILABLE." 13.3 IN PARTICULAR, GOTPETSONLINE.COM™, ITS SUBSIDIARIES AND AFFILIATES, AND ITS LICENSORS DO NOT REPRESENT OR WARRANT TO YOU THAT ANY OF THE FOLLOWING ARE TRUE: (a) YOUR USE OF THE SERVICES WILL MEET YOUR REQUIREMENTS. (b) YOUR USE OF THE SERVICES WILL BE UNINTERRUPTED, TIMELY, SECURE OR FREE FROM ERROR. (c) ANY INFORMATION OBTAINED BY YOU AS A RESULT OF YOUR USE OF THE SERVICES WILL BE ACCURATE OR RELIABLE. (d) THAT DEFECTS IN THE OPERATION OR FUNCTIONALITY OF ANY SERVICES PROVIDED TO YOU WILL BE CORRECTED. (e) ANY WEBSITE OR ADS CREATED WITH GOTPETSONLINE.COM™ TECHNOLOGY SATISFIES THE REQUIREMENTS PROMULGATED BY ANY REGULATORY BOARD OR AGENCY, OR STATE OR FEDERAL LAW THAT GOVERN WEBSITE, ADVERTISING OR OTHER INFORMATION PUBLICATION IN YOUR INDUSTRY. 13.4 ANY MATERIAL DOWNLOADED OR OTHERWISE OBTAINED THROUGH THE USE OF THE SERVICES IS DONE AT YOUR OWN DISCRETION AND RISK AND THAT YOU WILL BE SOLELY RESPONSIBLE FOR ANY DAMAGE TO YOUR COMPUTER SYSTEM OR OTHER DEVICE OR LOSS OF DATA THAT RESULTS FROM THE DOWNLOAD OF ANY SUCH MATERIAL. 13.5 NO ADVICE OR INFORMATION, WHETHER ORAL OR WRITTEN, OBTAINED BY YOU FROM GOTPETSONLINE.COM™ OR THROUGH OR FROM THE SERVICES, SHALL CREATE ANY WARRANTY NOT EXPRESSLY STATED IN THE AGREEMENT. 13.6 GOTPETSONLINE.COM™ FURTHER EXPRESSLY DISCLAIMS ALL WARRANTIES AND CONDITIONS OF ANY KIND, WHETHER EXPRESS OR IMPLIED, INCLUDING, BUT NOT LIMITED TO THE IMPLIED WARRANTIES AND CONDITIONS OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NON-INFRINGEMENT. 13.7 For information about GOTPETSONLINE.com™ payment system software, please read Payment System Software of GOTPETSONLINE.com™, hereinafter referred to as "Payment System Software", and incorporated by reference herein. You agree to the provisions of the Payment System Software section.
Section 14: Limitation of Liability 14.1 SUBJECT TO OVERALL PROVISION IN PARAGRAPH 13.1 ABOVE, YOU EXPRESSLY UNDERSTAND AND AGREE THAT GOTPETSONLINE.COM™ AND THE INDEMNIFIED PARTIES SHALL NOT BE LIABLE TO YOU FOR ANY OF THE FOLLOWING: (a) ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL CONSEQUENTIAL OR EXEMPLARY DAMAGES WHICH MAY BE INCURRED BY YOU, HOWEVER CAUSED AND UNDER ANY THEORY OF LIABILITY. THIS SHALL INCLUDE, BUT NOT BE LIMITED TO, ANY LOSS OF PROFIT (WHETHER INCURRED DIRECTLY OR INDIRECTLY), ANY LOSS OF GOODWILL OR BUSINESS REPUTATION, ANY LOSS OF DATA SUFFERED, COST OF PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES, OR OTHER INTANGIBLE LOSS. (b) ANY LOSS OR DAMAGE WHICH MAY BE INCURRED BY YOU, INCLUDING BUT NOT LIMITED TO LOSS OR DAMAGE AS A RESULT OF ANY OF THE FOLLOWING: (i) ANY RELIANCE PLACED BY YOU ON THE COMPLETENESS, ACCURACY OR EXISTENCE OF ANY ADVERTISING, OR AS A RESULT OF ANY RELATIONSHIP OR TRANSACTION BETWEEN YOU AND ANY ADVERTISER OR SPONSOR WHOSE ADVERTISING APPEARS ON THE SERVICES; OR WHOSE WEBSITES ARE MADE AND/OR HOSTED BY GOTPETSONLINE.COM™. (ii) ANY CHANGES WHICH GOTPETSONLINE.COM™ MAY MAKE TO THE SERVICES, OR FOR ANY PERMANENT OR TEMPORARY CESSATION IN THE PROVISION OF THE SERVICES (OR ANY FEATURES WITHIN THE SERVICES). (iii) THE DELETION OF, CORRUPTION OF, OR FAILURE TO STORE, ANY CONTENT AND OTHER COMMUNICATIONS DATA MAINTAINED OR TRANSMITTED BY OR THROUGH YOUR USE OF THE SERVICES. (iv) YOUR FAILURE TO PROVIDE GOTPETSONLINE.COM™ WITH ACCURATE ACCOUNT INFORMATION. (v) YOUR FAILURE TO KEEP YOUR PASSWORD OR ACCOUNT DETAILS SECURE AND CONFIDENTIAL. (vi) YOUR FAILURE TO COMPLY WITH ANY REGULATORY BOARD OR AGENCY, OR STATE OR FEDERAL LAW THAT GOVERNS WEBSITE, ADVERTISING OR OTHER INFORMATION PUBLICATION IN YOUR INDUSTRY. 14.2 GOTPETSONLINE.COM™ MAY PROVIDE, OR THIRD-PARTIES MAY PROVIDE, LINKS TO OTHER WEBSITES. (a) YOU AGREE THAT GOTPETSONLINE.COM™ IS NOT RESPONSIBLE FOR THE AVAILABILITY OF SUCH EXTERNAL WEBSITES, AND DOES NOT ENDORSE AND IS NOT RESPONSIBLE OR LIABLE FOR ANY CONTENT, ADVERTISING, PRODUCTS, OR OTHER MATERIALS ON OR AVAILABLE FROM SUCH WEBSITES. (b) YOU FURTHER ACKNOWLEDGE AND AGREE THAT GOTPETSONLINE.COM™ SHALL NOT BE RESPONSIBLE OR LIABLE FOR ANY DAMAGE OR LOSS CAUSED OR ALLEGED TO BE CAUSED BY OR IN CONNECTION WITH USE OF OR RELIANCE ON ANY SUCH CONTENT, GOODS OR SERVICES AVAILABLE ON OR THROUGH ANY SUCH WEBSITES. 14.3 GOTPETSONLINE.COM™ MAY BE USED AS A TOOL TO CREATE OTHER WEBSITES AND MAY HOST OTHER WEBSITES. (a) YOU AGREE THAT GOTPETSONLINE.COM™ IS NOT RESPONSIBLE FOR THE AVAILABILITY OF SUCH EXTERNAL WEBSITES, AND DOES NOT ENDORSE AND IS NOT RESPONSIBLE OR LIABLE FOR ANY CONTENT, ADVERTISING, PRODUCTS, OR OTHER MATERIALS ON OR AVAILABLE FROM SUCH WEBSITES. (b) YOU FURTHER ACKNOWLEDGE AND AGREE THAT GOTPETSONLINE.COM™ SHALL NOT BE RESPONSIBLE OR LIABLE FOR ANY DAMAGE OR LOSS CAUSED OR ALLEGED TO BE CAUSED BY OR IN CONNECTION WITH USE OF OR RELIANCE ON ANY SUCH CONTENT, GOODS OR SERVICES AVAILABLE ON OR THROUGH ANY SUCH WEBSITES. 14.4 THE LIMITATIONS ON GOTPETSONLINE.COM™ LIABILITY TO YOU IN PARAGRAPH 14.1 ABOVE SHALL APPLY WHETHER OR NOT GOTPETSONLINE.COM™ HAS BEEN ADVISED OF OR SHOULD HAVE BEEN AWARE OF THE POSSIBILITY OF ANY SUCH LOSSES ARISING.
Section 15: Account Security and Passwords 15.1 You agree and understand that you are responsible for maintaining the confidentiality of passwords associated with any account you use to access the Services. 15.2 Accordingly, you agree that you will be solely responsible to GOTPETSONLINE.com™ for all activities that occur under your account. 15.3 If you become aware of any unauthorized use of your password or of your account, you agree to notify GOTPETSONLINE.com™ immediately as provided on the Contact Us page or Support pages at GOTPETSONLINE.com™.
Section 16: Service Updates 16.1 Some of the Services which you use may be updated from time to time from GOTPETSONLINE.com™. These updates are designed to improve, enhance and further develop the Services and may take the form of bug fixes and enhanced functions.
Section 17: Indemnity 17.1 You agree to indemnify and hold GOTPETSONLINE.com™ and the Indemnified Parties, which includes all Subsidiaries and Affiliated Legal Entities, harmless from any claim or demand, including reasonable attorneys' fees, made by any third-party due to or arising out of Content you submit, transmit or make available through our Services, your use of the Services, your connection to our Services, your violation of the Agreement, or your violation of any rights of another. 17.2 More specifically, you agree to indemnify and hold GOTPETSONLINE.com™ and the Indemnified Parties, which includes all Subsidiaries and Affiliated Legal Entities, harmless from any and all claims and demands, losses, liability costs and expenses (including, but not limited to, reasonable attorneys' fees), incurred by an Indemnified Party arising out of or related to any of the following: (a) Your breach of this Agreement. (b) Any information (including but not limited to your Content and your publicly posted information) submitted, posted, or otherwise provided by you at your website and/or to GOTPETSONLINE.com™ and/or its affiliates. (c) Any dispute or litigation between an Indemnified Party and a third-party caused by your actions. (d) Your negligence or violation or alleged violations of any rights of another. (e) Any third-party claim against GOTPETSONLINE.com™ or the Indemnified Parties, which includes all Subsidiaries and Affiliated Legal Entities, for any of the following: (i) Personal injury or property damage to the extent such Liabilities arise out of or result from the negligence or other tortuous conduct of Customer. (ii) The breach of any representation or warranty made by Customer. (iii) Any claim by a customer of Customer against any Indemnified Party to the extent such liabilities arise out of or result from customer's business dealings with such Customer, including, without limitation the provision of any Services to such customer. 17.3 These obligations will survive any termination of your relationship with GOTPETSONLINE.com™ or your use of the Services. To the fullest extent permitted by law, the foregoing indemnity will apply regardless of any fault, negligence, or breach of warranty or contract of GOTPETSONLINE.com™ and the Indemnified Parties, which includes all Subsidiaries and Affiliated Legal Entities.
Section 18: Agreement Termination 18.1 The Agreement will continue to apply until terminated by either you or GOTPETSONLINE.com™ as set out below. 18.2 If you want to terminate your Agreement with GOTPETSONLINE.com™ you may do so by any of the following: (a) Notifying GOTPETSONLINE.com™ at any time by sending such notice in writing to the GOTPETSONLINE.com™ address as provided on the Contact Us or Support pages of GOTPETSONLINE.com™ as set out at the beginning of this Agreement. (b) Closing your accounts for all of the Services which you use, where GOTPETSONLINE.com™ has made this option available to you. 18.3 GOTPETSONLINE.com™ may at any time, terminate its Agreement with you if any of the following occurs: (a) You have breached any provision of the Agreement (or have acted in a manner which clearly shows that you do not intend to, or are unable to comply with the provisions of the Agreement). (b) GOTPETSONLINE.com™ is required to do so by law (for example, where the provision of the Services to you is, or becomes, unlawful). (c) The partner with whom GOTPETSONLINE.com™ offered the Services to you has terminated its relationship with GOTPETSONLINE.com™ or ceased to offer the Services to you. (d) GOTPETSONLINE.com™ is transitioning to no longer providing the Services to users in the country in which you are resident or from which you use the Service. (e) The provision of the Services to you by GOTPETSONLINE.com™ is, in the opinion of GOTPETSONLINE.com™, no longer commercially viable. 18.4 In addition to the aforementioned reasons for termination, you agree that GOTPETSONLINE.com™, in its sole discretion, may terminate your password, account (or any part thereof) or use of our Service, and remove and discard any Content within our Service, for any other reason, including, without limitation, for lack of use or if GOTPETSONLINE.com™ believes that you have violated or acted inconsistently with the letter or spirit of the Agreement. (a) GOTPETSONLINE.com™ may also and at any time discontinue providing our Service with or without notice. (b) You agree that any termination of your access to our Service under any provision of this Agreement may be effected without prior notice, and acknowledge and agree that GOTPETSONLINE.com™ may immediately deactivate or delete your account and all related information and files in your account and/or bar any further access to such files or our Service. (c) Further, you agree that GOTPETSONLINE.com™ shall not be liable to you or any third-party for any termination of your access to our Service. 18.5 Nothing in this Section shall affect GOTPETSONLINE.com™ rights regarding provision of Services under Section 7 of the Agreement. 18.6 When this Agreement comes to an end, all of the legal rights, obligations and liabilities that you and GOTPETSONLINE.com™ have benefited from, been subject to (or which have accrued over time while the Agreement has been in force) or which are expressed to continue indefinitely, shall be unaffected by this cessation, and the provisions of Paragraph 20.13 shall continue to apply to such rights, obligations and liabilities indefinitely. 18.7 If you cancel this service within seven days of initial purchase, then GOTPETSONLINE.com™ will issue a full refund. (a) If you cancel this service after seven days or after any subsequent monthly purchases of services or upgrades, then GOTPETSONLINE.com™ will not issue a refund, either whole or in part; however GOTPETSONLINE.com™ will cease billing you for any billing periods thereafter. GOTPETSONLINE.com™ provides services on a month-to-month basis. (b) You may cancel at any time by logging in to and canceling your account. (c) In other words, once you cancel you will not be charged again, but you are responsible for whatever charges have already been incurred for the current billing period. (d) For example, if your billing cycle is on the 18th of every month, and you cancel on the 24th, you will still have to pay for the current month (which you would likely already have done), but you will not be charged again after that.
Section 19: Agreement Language 19.1 Where GOTPETSONLINE.com™ has provided you with a translation of the English language version of the Agreement, then you agree that the translation is provided for your convenience only and that the English language version of the Agreement will govern your relationship with GOTPETSONLINE.com™. 19.2 If there is any contradiction between what the English language version of the Agreement says and what a translation says, then the English language version shall take precedence.
Section 20: Miscellaneous 20.1 When you use the Services, you may sometimes (as a result of, or through your use of the Services) use a service or download a piece of software, or purchase goods, which are provided by another person or company. (a) Your use of these other services, software or goods may be subject to separate terms between you and the company or person concerned. (b) If so, the Agreement does not affect your legal relationship with these other companies or individuals. 20.2 The Agreement constitutes the whole legal agreement between you and GOTPETSONLINE.com™ and governs your use of the Services (but excluding any services which GOTPETSONLINE.com™ may provide to you under a separate written agreement), and completely replaces any prior agreements between you and GOTPETSONLINE.com™ in relation to the Services. 20.3 You agree that GOTPETSONLINE.com™ may provide you with notices, including those regarding changes to the Agreement, by email, regular mail, or postings on the Services. 20.4 You agree that if GOTPETSONLINE.com™ does not exercise or enforce any legal right or remedy which is contained in the Agreement (or which GOTPETSONLINE.com™ has the benefit of under any applicable law), this will not be taken to be a formal waiver of GOTPETSONLINE.com™ rights and that those rights or remedies will still be available to GOTPETSONLINE.com™. 20.5 If any court of law, having the jurisdiction to decide on this matter, rules that any provision of this Agreement is invalid, and said ruling is not appealed, then that provision will be removed from the Agreement for the jurisdiction in which the ruling took place without affecting the rest of the Agreement. The remaining provisions of the Agreement will continue to be valid and enforceable. 20.6 You agree that you shall not assign this Agreement or any rights or obligations hereunder, by operation of law or otherwise, without prior written approval of GOTPETSONLINE.com™, and any such attempted assignment shall be void. GOTPETSONLINE.com™ shall have the right to freely assign this Agreement, and its rights and obligations hereunder, to any third-party without requiring your consent or notice. Subject to the foregoing, this Agreement shall be binding upon and inure to the benefits of the parties hereto, their successors and permitted assigns. 20.7 The section and subsection titles in the Agreement are for convenience only and have no legal or contractual effect. 20.8 Each party is an independent contractor and not an agent or representative of any other party. 20.9 No party shall have any right or authority to create any obligation or make any representation or warranty in the name or on behalf of any other party. 20.10 This Agreement shall not be interpreted or construed to create an association, joint venture or partnership between the parties or to impose any partnership obligation or liability upon any party. 20.11 The parties acknowledge and agree that this Agreement may be entered into electronically, and without the necessity of written signatures. 20.12 You acknowledge and agree that each member of the group of companies of which GOTPETSONLINE.com™ is the parent shall be third-party beneficiaries to the Agreement and that such other companies shall be entitled to directly enforce, and rely upon, any provision of the Agreement which confers a benefit on (or rights in favor of) them. Other than this, no other person or company shall be third-party beneficiaries to the Agreement. 20.13 You agree that the Agreement, and your relationship with GOTPETSONLINE.com™ under the Agreement, shall be governed by the laws of the State of Texas without regard to its conflict of laws provisions. You and GOTPETSONLINE.com™ agree to submit to the exclusive jurisdiction of the courts located within the county of Dallas, Texas to resolve any legal matter arising from the Agreement. Notwithstanding this, you agree that GOTPETSONLINE.com™ shall still be allowed to apply for injunctive remedies (or an equivalent type of urgent legal relief) in any jurisdiction.
Section 21: Arbitration 21.1 Except as expressly set forth herein, all disputes, claims and controversies between you and GOTPETSONLINE.com™, relating to or arising out of the Agreement, the rights and obligations of an Affiliate, Client, or GOTPETSONLINE.com™ or any other claims or causes of action relating to the performance of our Services under the Agreement shall be settled totally and finally by arbitration in Dallas County, Texas or such other location as GOTPETSONLINE.com™ prescribes, in accordance with this Agreement and the Commercial Arbitration Rules of the American Arbitration Association. Except as expressly set forth herein, arbitration shall be the exclusive method available for resolution of such claims and disputes. 21.2 There shall be one arbitrator who shall be an attorney who shall have expertise in business law transactions, and preferably an attorney knowledgeable in the direct selling industry. GOTPETSONLINE.com™ shall select the arbitrator at its sole discretion from the panel which the American Arbitration Association provides. Each party to the arbitration shall be responsible for its own costs and expenses of arbitration, including legal and filing fees. If an Affiliate or Client files a claim or counterclaim against GOTPETSONLINE.com™, an Affiliate or Client shall do so on an individual basis and not with any other Affiliate or Client or as part of a class action. The arbitrator shall have the right in Affiliate's or Client's discretion to authorize the obtaining of discovery, including the taking of depositions of witnesses for the purpose of discovery. The presentations of parties in the arbitration proceeding shall be commenced and completed within sixty (60) days after the selection of the arbitrator and the arbitrator shall render arbitrator's decision in writing within thirty (30) days after the completion of such presentations. The decision of the arbitrator shall be final and binding on the parties and may, if need be, be reduced to a judgment in any court of competent jurisdiction. At the request of any party, the arbitrator shall make and provide to the parties written findings of fact and conclusions of law. This agreement to arbitration shall survive any termination or expiration of the Agreement. 21.3 Notwithstanding the foregoing, the arbitrator shall have no jurisdiction over disputes relating to the ownership, validity, use or registration of any mark, copyright, or other intellectual property or proprietary or confidential information of GOTPETSONLINE.com™ without the prior written consent of GOTPETSONLINE.com™. GOTPETSONLINE.com™ may seek any applicable remedy in any applicable forum with respect to these disputes and with respect to money owing to GOTPETSONLINE.com™. In addition to monetary damages, GOTPETSONLINE.com™ may obtain injunctive relief against an Affiliate or Client for any violation of the Agreement or misuse of GOTPETSONLINE.com™ trademarks, copyrights or confidential information. 21.4 Nothing in this Agreement shall prevent GOTPETSONLINE.com™ from terminating the Agreement or from applying to and obtaining from any court having jurisdiction a writ of attachment, a temporary injunction, preliminary injunction and/or other injunctive or emergency relief available to safeguard and protect GOTPETSONLINE.com™ interests prior to the filing of or during or following any arbitration or other proceeding or pending the handing down of a decision or award in connection with any arbitration or other proceeding. 21.5 Nothing contained herein shall be deemed to give the arbitrator any authority, power or right to alter, change, amend, modify, add to, or to subtract from any of the provisions of the Agreement.
Section 22: Acknowledgement 22.1 YOU ACKNOWLEDGE THAT YOU HAVE READ THESE CONDITIONS AND ALL RELATED WEBSITE SCREENS REFERENCED AND INCORPORATED IN THESE CONDITIONS AND YOU AGREE TO ALL ITS TERMS AND CONDITIONS. YOU HAVE INDEPENDENTLY EVALUATED ALL ASPECTS OF THESE CONDITIONS AND THE DESIRABILITY OF ENTERING INTO THE TRANSACTIONS CONTEMPLATED HEREIN AND ARE NOT RELYING ON ANY REPRESENTATION, GUARANTEE OR STATEMENT OTHER THAN THOSE EXPRESSLY SET FORTH IN HEREIN.